Description
Monitor unstained living cell cultures inside deep culture flasks using the Optika IM-3 Inverted Trinocular Microscope. Operating with an infinity-corrected IOS optical system, it delivers 100x, 200x, and 400x phase-contrast magnification via three specialized Long Working Distance (LWD) W-PLAN PH objectives: PH 10x, PH 20x, and PH 40x. Because the objectives sit beneath the stage, researchers can inspect adherent cell lines directly through the plastic bottoms of Petri dishes and multi-well plates without disturbing liquid media.
Transmitted light is supplied by an overhead 8W white LED lamp block that mimics warm halogen contrast without cooking live cells, focused through an LWD pre-centered condenser (NA 0.30) with phase ring slider plates. A side-facing camera port with a sliding path toggle routes digital video directly to C-mount cameras while maintaining binocular viewing through high-eyepoint WF 10x/22 mm eyepieces. The oversized 250 x 160 mm stage plate includes modular glass and metal inserts, paired with a side-mounted mechanical XY guide (120 x 78 mm travel) across a 10.5 kg composite metal frame.
Key Features of Optika IM-3 Inverted Trinocular Microscope
A professional inverted biological trinocular microscope engineered for live-cell culture monitoring, oncology research, and transparent organism tracking. Combines three LWD W-PLAN phase-contrast objectives with an 8W LED transmitted lamp, side photo port, and a 120 x 78 mm mechanical stage.
- Inverted optical path allows direct inspection through flask and multi-well vessel bottoms
- IOS LWD W-PLAN PH infinity objectives: PH 10x, PH 20x, and PH 40x for living cells
- Overhead high-output 8W white LED lamp provides cool, halogen-equivalent contrast
- Long-working-distance pre-centered condenser (NA 0.30) with selectable phase slider cards
- Side-mounted vertical photo connection channel with integrated path selection sliding switch
- Extra-widefield high-eyepoint WF 10x/22 mm eyepieces on a 45-degree inclined trinocular head
- Oversized 250 x 160 mm mechanical stage plate with 120 x 78 mm side-mounted XY guide
- Complete 10.5 kg laboratory-grade composite frame isolates cultures from table vibration
Inverted Architecture & Living Cell Phase Contrast
Transparent living cells, stem cell colonies, and protozoa lack natural pigmentation, appearing invisible under standard brightfield light. The W-PLAN phase optics and pre-centered condenser rings convert subtle refractive index shifts into sharp amplitude contrast, highlighting cell membranes, mitotic spindles, and apoptotic vacuoles without toxic stains. The LWD objective design compensates for vessel bottom thickness, keeping adherent monolayers tack-sharp.
Side-Mounted Camera Port & 8W Cool LED Illumination
Placing the camera port on the side of the microscope body keeps the vertical working space above the stage completely unobstructed, making it easy to maneuver tall culture bottles and microinjection needles. The 8W LED illuminator runs cool to the touch, preventing thermal currents from drying out open Petri dishes during extended time-lapse logging. The side-mounted mechanical stage guide allows smooth scanning across multi-well plates with fingertip control.
- Side camera channel preserves overhead clearance for tall culture bottles and tools
- 8W LED light engine eliminates thermal stress on living cellular specimens
- Pre-centered phase slider cards allow instant toggling between brightfield and phase modes
Optika Ten-Year Manufacturer Limited Warranty
Optika covers the IM-3 Phase Contrast microscope with an industry-leading 10-year warranty on mechanical and optical components, backed by a two-year warranty on electronic LED boards and dimmer controls. Inside the box: the inverted biological tower, three LWD phase objectives, phase ring slider cards, paired WF 10x/22 mm eyepieces, phase centering telescope, stage inserts, power cord, and user manual.
Optika IM-3 Inverted Trinocular Microscope Phase Contrast IOS LWD W-PLAN 100x-400x EU Specs
| Brand | Optika |
| Full Model Code / SKU | IM-3 Phase Contrast |
| Country of Origin | Italy |
| Microscope Type | Professional Inverted Biological Trinocular Microscope with Advanced Phase Contrast Verification Loops |
| Optical System Type | IOS Infinity Corrected Optical System providing distortion-free tracking profiles across wide structural fields |
| Observation Tube Type | Trinocular observation assembly combining dual visual ocular ports with a side-facing video-imaging line |
| Tube Inclination & Rotation | Ocular viewing barrels are inclined at a comfortable 45-degree angle with adjustable interpupillary spacing distances |
| Eyepiece / Field of Number | Pair of extra-widefield high-eyepoint WF10x eyepieces displaying a large field-of-view index of exactly 22 mm |
| Nosepiece Design | Quintuple revolving nosepiece operating on internal precision ball-bearings with structural click-stop positioning |
| Objectives Classification | IOS LWD W-PLAN PH Professional Long-Working-Distance Infinitely Corrected Phase Contrast Objectives |
| Objectives Magnification Breakdown | Includes three specialized lenses: IOS LWD W-PLAN PH 10x, PH 20x, and a high-performance PH 40x objective cartridge |
| Total Magnification Range | Provides discrete compound cell-structure magnification verification boundaries at 100x, 200x, and up to 400x points |
| Focusing Type & Graduation | Coaxial coarse and fine adjustment tracker wheels fitted with safety stop brackets; fine tracking increments at 0.002 mm |
| Stage Dimensions & Surface Coating | Oversized flat mechanical fixed stage base measuring 250 x 160 mm with modular transparent glass and metal stage inserts |
| Stage Travel Range & Controls | Side-mounted mechanical stage control guide system delivering an adjustable structural reach span of 120 x 78 mm |
| Condenser Type & Numerical Aperture | Long-working-distance pre-centered condenser assembly tracking a 0.30 N.A. value with slider slot phase ring plates |
| Illumination Transmitted Type | Overhead mounted high-output 8W white LED lamp block mimicking traditional warm halogen contrast profiles |
| Illumination Epi-EPI/Reflected | None |
| Light Intensity Management | Continuous electronic brightness wheel potentiometer tracker matching complex living tissue transparency settings |
| Camera Port / C-Mount Adapter Split | Side-mounted photo connection channel containing an integrated path selection sliding switch layout toggle |
| Contrast Methods Capability | Transmitted brightfield microscopy, advanced living cell phase contrast tracking, cellular morphology analysis |
| Power Supply & Voltage Input | Standard integrated European specification multi-voltage internal power transformer accepting 100-240V AC inputs |
| Physical Dimensions & Net Weight | Chassis height 495 mm, width 250 mm, depth 500 mm, laboratory grade composite structural mass exactly 10.5 kg |
| Standard Package Accessories Included | Inverted biological tower, three phase objectives, phase ring slider cards, dual eyepieces, telescope centering eyepiece |
| Primary Application & Environment | Live cell culture monitoring labs, oncology research facility counters, microbiology transparent organism tracking cells |




















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