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Description
Screen for tuberculosis and evaluate immunofluorescence antigens using the Optika B-510FL Trinocular Fluorescence Microscope. It features an external 100W HBO high-pressure mercury vapor lamp house paired with pre-installed Blue (FITC/acridine orange) and Green (TRITC/rhodamine) excitation filter cubes. Operating on an infinity-corrected IOS optical system, it delivers 40x, 100x, and 400x magnification via three IOS W-PLAN Plan-Achromatic objectives: 4x/0.10, 10x/0.25, and a high-transmission spring-loaded 40x/0.65 lens designed specifically for fluorescence signal clarity without autofluorescence flare.
Transmitted brightfield light is supplied by an 8W white X-LED8 Köhler illuminator with an adjustable field iris diaphragm and a swing-out Abbe condenser (NA 1.25). The 30-degree inclined trinocular head incorporates an overhead photo port with a selectable 100:0 visual / 0:100 camera beam splitter, paired with extra-widefield high-eyepoint WF 10x/22 mm eyepieces. The double-layer 233 x 147 mm mechanical stage provides 78 x 54 mm of low-drop coaxial XY travel, supported by a 10.5 kg cast chassis (270 x 390 x 435 mm) with 2-micron coaxial focus graduations on universal 100-240V AC power with an external mercury ballast console.
Key Features of Optika B-510FL Trinocular Fluorescence Microscope
A research-grade compound epi-fluorescence trinocular microscope featuring a 100W HBO mercury vapor light engine and dual Blue/Green filter cubes. Combines three IOS W-PLAN objectives with an 8W X-LED8 Köhler illuminator, swing-out Abbe condenser, and a dedicated vertical camera port.
- IOS W-PLAN plan-achromatic infinity objectives: 4x, 10x, and high-transmission spring-loaded 40x
- High-pressure 100W HBO mercury vapor lamp house with external arc ballast power supply
- Pre-installed Blue and Green fluorescence excitation cubes for FITC, GFP, and TRITC tagging
- Full Köhler transmitted illumination powered by an energy-efficient 8W X-LED8 white LED module
- Dedicated vertical photo port tower with selectable 100:0 / 0:100 optical beam splitter
- Extra-widefield high-eyepoint WF 10x/22 mm eyepieces on a 30-degree inclined trinocular head
- Oversized 233 x 147 mm double-layer mechanical stage with 78 x 54 mm coaxial XY drive
- Solid 10.5 kg structural composite frame provides complete vibration-free benchtop stability
100W HBO Mercury Light Engine & Fluorophore Excitation
Faint antibody signals and low-copy pathogens require high-density mercury spectral emission peaks to fluoresce cleanly. The 100W HBO arc lamp directs intense excitation rays through the Blue and Green filter blocks, causing tagged cellular markers to glow brightly against a pitch-black background. The external ballast console regulates arc voltage to prevent flicker, while the IOS W-PLAN optics preserve flat-field edge sharpness across the entire 22 mm field of view.
Full Köhler 8W X-LED8 & Trinocular Camera Output
Swapping between epi-fluorescence and standard brightfield pathology takes a flick of a lever: engage the sub-stage 8W X-LED8 to review cellular morphology under cool, daylight-balanced light without cooking live specimens. The swing-out Abbe condenser ensures uniform illumination across all magnifications, while the vertical photo port routes 100% of the light signal to digital C-mount cameras for low-noise documentation of patient slides.
- High-peak 100W HBO burner provides maximum signal-to-noise ratio on faint fluorophores
- Swing-out Abbe condenser matches light cones to low-power 4x overview scans and 40x passes
- Selectable beam splitter allows 100% light transfer to digital cameras for low-noise capture
Optika Ten-Year Manufacturer Limited Warranty
Optika backs the B-510FL microscope body and optical train with an industry-leading 10-year warranty, covering internal LED circuitry and the external 100W mercury power console for two years. The complete factory package contains the trinocular frame tower, three W-PLAN objectives, HBO lamp house, mercury burner bulb, Blue and Green filter cubes, external power supply, dust wrap, and user manual.
Optika B-510FL Fluorescence Trinocular Microscope FL-HBO B & G Filter Set W-PLAN IOS 40x-400x Specs
| Brand | Optika |
| Full Model Code / SKU | B-510FL |
| Country of Origin | Italy |
| Microscope Type | Advanced Epi-Fluorescence Trinocular Compound Microscope with High-Pressure HBO Mercury Vapor Engine |
| Optical System Type | IOS Infinity Corrected Optical System providing distortion-free tracking profiles across wide structural fields |
| Observation Tube Type | Trinocular observation assembly combining dual visual ocular ports with an overhead vertical photo-imaging line |
| Tube Inclination & Rotation | Ocular viewing barrels are inclined at a comfortable 30-degree angle with adjustable interpupillary spacing distances |
| Eyepiece / Field of Number | Pair of extra-widefield high-eyepoint WF10x eyepieces displaying an oversized field index of exactly 22 mm |
| Nosepiece Design | Quintuple revolving nosepiece operating on internal precision ball-bearings with structural click-stop positioning |
| Objectives Classification | IOS W-PLAN Infinitely Corrected Plan-Achromatic Lenses calibrated for uniform flat fields and linear transmission |
| Objectives Magnification Breakdown | Includes three high-grade lenses: IOS W-PLAN 4x, 10x, and a specialized high-transmission 40x (Spring) cartridge |
| Total Magnification Range | Delivers discrete structural compound magnification checkpoints tracking through 40x, 100x, and up to 400x points |
| Focusing Type & Graduation | Coaxial coarse and fine adjustment tracker wheels fitted with safety stop brackets; fine tracking increments at 0.002 mm |
| Stage Dimensions & Surface Coating | Oversized double-layer mechanical sliding specimen stage measuring 233 x 147 mm with a wear-resistant black finish coat |
| Stage Travel Range & Controls | Coaxial drop-down X-Y directional adjustment tracks delivering an active cross-travel span of exactly 78 x 54 mm |
| Condenser Type & Numerical Aperture | Swing-out Abbe condenser assembly tracking a maximum 1.25 N.A. index value, fitted with a custom iris diaphragm |
| Illumination Transmitted Type | Patented X-LED8 system featuring a high-power 8W white LED module paired with a custom field iris layout for full Koehler setup |
| Illumination Epi-EPI/Reflected | 100W high-pressure HBO mercury vapor lamp housing assembly coupled with standard Blue (B) and Green (G) filter blocks |
| Light Intensity Management | Independent electronic dimming controls managing transmitted white LED and external power supply transformer for HBO lamp |
| Camera Port / C-Mount Adapter Split | Dedicated vertical photo port tower featuring a selectable light path splitter configuration (100% visual / 100% video) |
| Contrast Methods Capability | High-intensity HBO mercury fluorescence microscopy, transmitted brightfield analysis, rapid cellular antigen verification |
| Power Supply & Voltage Input | Standard multi-voltage internal power line for frame accepting 100-240V AC; external ballast box for mercury arc line |
| Physical Dimensions & Net Weight | Chassis height 435 mm, width 270 mm, depth 390 mm, laboratory grade composite structural mass exactly 10.5 kg |
| Standard Package Accessories Included | Trinocular frame tower, three W-PLAN lenses, HBO lamp house, mercury burner bulb, Blue/Green cubes, power adapters |
| Primary Application & Environment | Medical pathology counting centers, advanced tuberculosis diagnostic labs, academic research biological workspaces |
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