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Description
Operating with four discrete excitation channels and a 100W halogen epi-illuminator, the Kern OBN 148 Trinocular Fluorescence Microscope delivers multi-stain nuclear mapping across advanced pathology and oncology suites. Powered by an infinity-corrected optical system with true flat-field alignment, it spans 40x, 100x, 200x, 400x, and 1000x magnification via five Plan Achromatic objectives: 4x/0.10, 10x/0.25, 20x/0.40, spring-loaded 40x/0.65, and spring-loaded 100x/1.25 oil immersion, optimized for broad-spectrum transmission across deep UV, violet, and visible wavelengths.
Epi-fluorescence is driven by an external 12V/100W halogen housing and a 4-position turret pre-installed with Blue (FITC/GFP), Green (TRITC), UV (DAPI/Hoechst), and Violet filter sets. Transmitted brightfield utilizes full Köhler illumination with a 6V/20W halogen sub-stage module and a swing-out Abbe condenser (NA 0.9/1.25). The 30-degree inclined trinocular head features bilateral diopter equalization, high-eyepoint WF 10x/20 mm eyepieces, radiation scatter shielding, and a vertical 23.2 mm photo port. Built on a massive 15.8 kg chassis (395 x 550 x 440 mm), it features a 175 x 145 mm scratch-proof mechanical stage (78 x 55 mm XY travel) and 2-micron coaxial focus gears.
Key Features of Kern OBN 148 Trinocular Fluorescence Microscope
A flagship 4-channel reflected-light fluorescence and Köhler brightfield microscope designed for multi-stain oncology and cytogenetic discovery. Features five Infinity Plan objectives, a 100W halogen epi-illuminator, 4-position filter turret (Blue/Green/UV/Violet), and a 175 x 145 mm stage.
- Five Infinity Plan Achromatic objectives: 4x, 10x, 20x, spring-loaded 40x, and 100x oil
- 4-position excitation filter turret pre-installed with Blue, Green, UV, and Violet filter sets
- External 12V/100W halogen epi-fluorescence lamp housing with stabilized transformer block
- Full Köhler transmitted illumination with 6V/20W halogen bulb and swing-out Abbe condenser
- Vertical 23.2 mm photo port channel with beam splitter for digital camera integration
- Oversized 175 x 145 mm scratch-proof mechanical stage with 78 x 55 mm coaxial XY travel
- Coaxial coarse and fine focus drive with calibrated 2-micrometer fine division markings
- Solid 15.8 kg cast-metal chassis with integrated radiation scatter protection shield
4-Channel Filter Turret & Multi-Wavelength Excitation
Complex cytogenetic assays require rapid switching between nuclear stains like DAPI and antibody tags like FITC or TRITC. The 4-position rotating turret holds Blue, Green, UV, and Violet excitation blocks simultaneously, letting researchers transition between fluorophore channels in a fraction of a second without unseating hardware. The 100W halogen light engine delivers broad-spectrum excitation without the explosive arc hazards or warm-up delays associated with high-pressure burners.
Full Köhler Brightfield & 15.8kg Heavy-Duty Chassis
Setting up transmitted Köhler illumination is simple with the swing-out Abbe condenser and field diaphragm, producing homogeneous, glare-free brightfield light that defines stained tissue morphology cleanly. The 15.8 kg metal frame dampens building vibrations during high-power oil immersion passes, while the scratch-proof stage surface withstands aggressive daily slide swaps. Low-slung coaxial XY stalks and 2-micron focus knobs keep hand movements relaxed during extended evaluations.
- 4-channel filter wheel allows immediate multi-fluorophore nuclear and antibody mapping
- Swing-out Abbe condenser matches light cones to low-power 4x and high-power 100x lenses
- Integrated radiation shield protects technician eyes from stray reflected excitation light
Kern & Sohn Three-Year Laboratory Warranty
Kern & Sohn protects the OBN 148 with a comprehensive three-year factory warranty covering optical elements, stand castings, and mechanical stage drives, backed by two years on electrical halogen transformers and filter turrets. The complete package contains the OBN quintuple stand frame, external 100W halogen lamp house, 4-channel filter turret with Blue/Green/UV/Violet blocks, radiation shield, five Infinity Plan objectives, pair of WF 10x/20 mm eyepieces, photo port, immersion oil, power cables, dust cover, and manual.
Kern & Sohn OBN 148 Trinocular Fluorescence Microscope Infinity Plan 4x/10x/20x/40x/100x WF10x20 100W Halogen Blue/Green/UV/Violet Filterset Specs
| Brand | Kern & Sohn |
| Full Model Code / SKU | OBN 148 (Four-Channel Halogen Fluorescence Variant) |
| Country of Origin | Germany |
| Microscope Type | Premium Research-Grade Trinocular 4-Channel Reflected Light Fluorescence and Brightfield Microscope System |
| Optical System Type | Premium Infinity Corrected Optical Pathway Design System (True Flat-Field Alignment) |
| Observation Tube Type | Trinocular Head Unit with vertical photo dock port and integrated radiation scatter protection shielding |
| Tube Inclination & Rotation | 30° inclined viewing track, 360° rotatable head, adjustable individual interpupillary controls 50-75mm; diopter adjustment on both left and right eyepiece tubes |
| Eyepiece / Field of Number | WF 10x / 20 mm (Paired set, wide-field high-eyepoint layout finish) |
| Nosepiece Design | Revolving quintuple (5-position) reverse-facing objective turret housing mounted on heavy ball-bearing race tracking |
| Objectives Classification | Infinity Plan Achromatic High-Resolution Objective Lens Line (Anti-fungus treated, optimized for broad spectrum photon transmission across deep UV, violet, and visible fluorescence blocks) |
| Objectives Magnification Breakdown | Infinity Plan premium series tracking: 4x/0.10, 10x/0.25, 20x/0.40, 40x/0.65 spring-loaded, and 100x/1.25 spring-loaded oil-immersion micro pathways |
| Total Magnification Range | 40x, 100x, 200x, 400x, and 1000x multi-mode verification limits |
| Focusing Type & Graduation | Coaxial coarse and fine adjustment block knobs with adjustable tension control and safety stop; fine tracking graduation 2 micrometers |
| Stage Dimensions & Surface Coating | Professional oversized mechanical stage platform measuring approx. 175 x 145 mm with hard scratch-proof Gorilla-glass or anodized ceramic protective surface coating |
| Stage Travel Range & Controls | Precision coaxial X-Y axis mechanical translation range approx. 78 x 55 mm with low-positioned right-hand controls |
| Condenser Type & Numerical Aperture | Swing-out Abbe Condenser NA 0.9/1.25 with centering controls, field diaphragm control, and adjustable iris track |
| Illumination Transmitted Type | Full Koehler Illumination system utilizing an adjustable 6V 20W Halogen sub-stage module for perfectly centered brightfield tracing |
| Illumination Epi-EPI/Reflected | Epi-Fluorescence reflected light attachment block equipped with a heavy-duty 12V 100W Halogen light housing assembly and a 4-position turret holding excitation slider modules |
| Light Intensity Management | Independent dual electronic intensity control interfaces: base transmitted light dimmer wheel, heavy-duty 100W Halogen transformer control block for reflected pathways |
| Camera Port / C-Mount Adapter Split | Vertical photo port channel on top of head unit (23.2mm tube interface), standard beam-splitting path routing infrastructure |
| Contrast Methods Capability | Epi-fluorescence Blue excitation (FITC/GFP), Epi-fluorescence Green excitation (TRITC), Epi-fluorescence UV excitation (DAPI/Hoechst nuclear mapping), Epi-fluorescence Violet excitation (custom fluorophore tracking), premium flat-field Koehler brightfield mapping, digital image logging |
| Power Supply & Voltage Input | Base frame operates on 100-240V AC; external heavy-duty stabilized variable transformer injection module drives the high-output 100W reflected halogen bulb paths |
| Physical Dimensions & Net Weight | Base frame with lamp house extension 395 x 550 mm, height 440 mm, complete assembly net weight approx. 15.8 kg |
| Standard Package Accessories Included | OBN quintuple stand frame, external 100W Halogen lamp housing, internal 4-channel excitation turret filter wheel assembly (Blue, Green, UV, Violet filter sets pre-installed), light barrier shield, five infinity plan objectives, pair of WF 10x/20mm eyepieces, vertical photo channel, immersion oil flask, power cables, dust cover, manual |
| Primary Application & Environment | Comprehensive multi-stain pathology diagnostics offices, advanced nuclear mapping research units, oncology screening hubs tracking complex fluorophores, academic microbiology discovery blocks |
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