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Description
Map low-expression fluorophore tags across living cell lines using the Kern OCM 165 Inverted Microscope research platform. It combines an inverted 100W High-Pressure Mercury Vapor (HBO) epi-fluorescence illuminator with a 6V/30W halogen transmitted light path, delivering 100x, 200x, and 400x magnification. The under-stage quintuple nosepiece carries four Long Working Distance (LWD) Plan Achromatic objectives: 10x/0.25, 20x/0.40, and 40x/0.60 brightfield lenses, plus a specialized 20x/0.40 Phase Contrast (PH) objective for tracking unstained cellular morphology.
Epi-fluorescence excitation is managed via an integrated filter turret loaded with standard Blue and Green excitation filter cubes, powered by an external standalone HBO ballast console. The 45-degree inclined trinocular head features an inverted light distribution block (100:0 visual / 0:100 camera split) and a straight vertical photo tube, paired with high-eyepoint HWF 10x/22 mm eyepieces. The 210 x 241 mm stage features modular insert rings and a detachable universal mechanical specimen holder (120 x 80 mm XY travel) across a massive 21.0 kg cast frame with 2-micron coaxial focus graduations.
Key Features of Kern OCM 165 Inverted Microscope
A flagship inverted epi-fluorescence and phase contrast research microscope designed for molecular biology institutes, immunology labs, and live-cell imaging bays. Features a 100W HBO mercury illuminator, Blue/Green filter cubes, 30W halogen transmitted lighting, and a 120 x 80 mm mechanical stage.
- Inverted optical path accommodates living cell cultures inside Petri dishes and culture flasks
- 100W HBO high-pressure mercury vapor epi-illuminator with external ballast power box
- Integrated fluorescence filter turret loaded with standard Blue and Green excitation cubes
- Infinity Plan Achromatic LWD objectives: 10x, 20x, 40x, plus specialized 20x Phase Contrast
- LWD condenser (NA 0.30) provides 72 mm of vertical clearance for tall culture vessels
- Top-mounted vertical trinocular photo tube with selectable 100:0 / 0:100 optical beam splitter
- Large 210 x 241 mm stage with detachable mechanical specimen holder (120 x 80 mm XY travel)
- Massive 21.0 kg cast-metal chassis provides complete vibration-free benchtop stability
Dual-Source EPI-Fluorescence & LWD Phase Optics
Evaluating transfected cell cultures requires intense UV excitation alongside phase-contrast structural verification. The 100W mercury burner generates high-density excitation wavelengths that make GFP, FITC, and rhodamine markers glow intensely against dark cellular backgrounds. The LWD phase-contrast objective lets researchers verify cell viability and confluence in brightfield before switching to fluorescence, protecting live cells from photobleaching.
120x80mm Mechanical Stage & Trinocular Camera Output
The detachable mechanical stage guide provides smooth, precise coordinate translation across multi-well plates and culture flasks using drop-down right-hand stalks. The straight vertical photo port routes 100% of the light signal to digital C-mount cameras, ensuring low-noise digital image capture during faint fluorescence emission protocols. Coaxial coarse and fine focus knobs operate on internal vertical tracks with 2-micron fine division markings.
- Blue and Green filter cubes allow rapid toggling between standard immunofluorescence markers
- Selectable beam splitter allows 100% light transfer to digital cameras for low-noise capture
- Detachable XY mechanical stage provides 120 x 80 mm travel across micro-well plates
Kern & Sohn Three-Year Laboratory Warranty
Kern & Sohn protects the OCM 165 with a comprehensive three-year factory warranty covering optical elements, stand castings, and mechanical stage drives, backed by two years on electronic halogen circuitry and the external HBO ballast console. The complete package contains the OCM 165 frame stand, 100W HBO EPI-FL assembly attachment box, Blue & Green fluorescence filter cubes, external power box block, four Infinity Plan objectives, HWF 10x/22 mm eyepieces, filters, and manual.
Kern & Sohn OCM 165 Inverted Fluorescence Trinocular Microscope 100W HBO EPI-FL (B/G) Infinity Plan 10x/20x/40x/20PH WF10x22 30W Halogen Specs
| Brand | Kern & Sohn |
| Full Model Code / SKU | OCM 165 (Advanced Inverted Fluorescence System) |
| Country of Origin | Germany |
| Microscope Type | Professional Inverted EPI-Fluorescence and Phase Contrast Research Compound Microscope System |
| Optical System Type | Infinity Corrected Optical System (IOS) Lens Layout Path |
| Observation Tube Type | Trinocular Head Unit with inverted light distribution block (Standard 100:0 / 0:100 split routing paths) |
| Tube Inclination & Rotation | 45° inclined viewing track ergonomic setup with individual interpupillary tracking sliders (54-75 mm); diopter balance on both eye sleeves |
| Eyepiece / Field of Number | HWF 10x / 22 mm (High eye-point wide-field configuration, ideal for eyeglass wearers tracking sample overviews) |
| Nosepiece Design | Revolving quintuple (5-position) under-stage objective turret housing running on heavy-duty ball bearing tracks |
| Objectives Classification | Infinity Plan Achromatic Corrected Long Working Distance (LWD) and LWD Phase Contrast Objective Lens Configuration |
| Objectives Magnification Breakdown | Infinity Plan LWD series tracking paths: 10x/0.25, 20x/0.40, 40x/0.60 brightfield objectives, and a specialized 20x/0.40 Phase Contrast (PH) objective module |
| Total Magnification Range | 100x, 200x, and 400x professional observation structural increments |
| Focusing Type & Graduation | Coaxial coarse and fine adjustment block knobs working on stable vertical nosepiece elevation tracks; fine tracking graduation 2 micrometers |
| Stage Dimensions & Surface Coating | Large fixed mechanical stage platform measuring approx. 210 x 241 mm with modular insert rings, fitted with a heavy-duty detachable universal mechanical specimen holder frame layout |
| Stage Travel Range & Controls | X-Y axis translation layout measuring 120 x 80 mm tracking travel paths via low-positioned coaxial control knobs on the right-hand layout |
| Condenser Type & Numerical Aperture | Long Working Distance (LWD) Inverted Condenser structure tracking NA 0.3 (Working Distance 72 mm), fitted with an adjustable slider slider mechanism containing phase contrast rings |
| Illumination Transmitted Type | Powerful 6V/30W Halogen sub-stage overhead transmitted light module tracking uniform color profile paths across dense cell layers |
| Illumination Epi-EPI/Reflected | EPI-Fluorescence system assembly tracking 100W High-Pressure Mercury Vapor Lamp (HBO) source block pathways |
| Light Intensity Management | Dual control configuration: external standalone HBO power block supply for ignition control, base rotary rheostat for variable Halogen tracking paths |
| Camera Port / C-Mount Adapter Split | Top-mounted straight vertical trinocular photo tube port; requires model-specific C-mount adapter tracking camera integration logs |
| Contrast Methods Capability | Inverted Brightfield tracking, advanced Phase Contrast screening, Blue/Green (B/G) excitation filter channel EPI-Fluorescence immunofluorescence research analysis |
| Power Supply & Voltage Input | Dual connection: integrated transformer block module for 100-240V AC variable Halogen inputs, plus dedicated external controller block for the HBO lamp line links |
| Physical Dimensions & Net Weight | Base layout 350 x 250 mm (excluding external lamps), height 550 mm, complete assembly net weight approx. 21.0 kg |
| Standard Package Accessories Included | OCM 165 frame stand, 100W HBO EPI-FL assembly attachment box, Blue & Green fluorescence filter cube sets, external power box block, four Infinity Plan objectives, HWF 10x/22mm eyepieces, filters, manual |
| Primary Application & Environment | Molecular biology research institutes, advanced immunology workspaces, live-cell fluorescence imaging bays, genetic sorting laboratories, virology research clusters |
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