Description
The Optika B-293LD1 Fluorescence Trinocular Microscope delivers instant-on Blue LED excitation for tuberculosis auramine screening and cellular research. It features an upper epi-fluorescence attachment housing a high-output 3.5W Blue LED illuminator (450-490 nm excitation) paired with a Blue filter set, completely eliminating mercury burner warm-up delays and explosion hazards. Powered by an infinity-corrected IOS optical system, it delivers 40x, 100x, 400x, and 1000x magnification via four IOS N-PLAN flat-field objectives: 4x, 10x, spring-loaded 40x, and spring-loaded 100x oil immersion.
Transmitted brightfield light is supplied by an ultra-bright X-LED3 3.6W white LED system with custom condensing collectors, focused through an Abbe 1.25 NA condenser. The 30-degree inclined trinocular head incorporates a vertical photo port with a selectable 100:0 visual / 0:100 camera split path, paired with widefield high-eyepoint WF 10x/20 mm eyepieces. Its 150 x 139 mm double-layer mechanical stage features an enclosed belt-driven movement mechanism (75 x 33 mm XY travel), supported by a 7.5 kg cast chassis (235 x 290 x 420 mm) with coaxial 2-micron fine focus knobs running on universal 100-240V AC power.
Key Features of Optika B-293LD1 Fluorescence Trinocular Microscope
An accessible biological trinocular epi-fluorescence compound microscope featuring Blue LED excitation and a belt-driven mechanical stage. Combines four IOS N-PLAN objectives with an instant-on 3.5W Blue LED illuminator, 3.6W X-LED3 brightfield light, and a dedicated camera port.
- IOS N-PLAN flat-field infinity objectives: 4x, 10x, spring-loaded 40x, and 100x oil immersion
- High-output 3.5W Blue LED epi-fluorescence attachment with Blue filter set (450-490 nm)
- Instant-on LED operation eliminates mercury burner warm-up times and explosion hazards
- Patented X-LED3 3.6W white LED light platform delivers daylight-balanced brightfield illumination
- Innovative belt-driven 150 x 139 mm mechanical stage eliminates protruding gear racks
- Dedicated vertical photo port channel with selectable 100:0 / 0:100 optical beam splitter
- Independent electronic dimming controls for separate transmitted white and Blue LED light lines
- Solid 7.5 kg cast-metal chassis provides complete vibration-free benchtop stability
Blue LED Epifluorescence & Auramine Screening
Mercury vapor burners take 15 minutes to stabilize and must run continuously to avoid electrode damage. The solid-state 3.5W Blue LED on the B-293LD1 fires up instantly at full photon intensity, letting technicians screen emergency auramine smears without waiting. The narrow-band Blue emitter excites fluorochromes bound to mycobacterial cell walls, making bright green-gold bacilli stand out vividly against dark cellular debris without bulb degradation over time.
Belt-Driven Stage Safety & Trinocular Camera Output
Traditional mechanical stages feature sharp metal gear racks that stick out sideways, catching lab coat cuffs during repetitive work. The belt-driven stage on the B-293LD1 moves smoothly on internal belts, providing whisper-quiet translation with zero backlash across a 75 x 33 mm range. Engaging the vertical photo port slider directs 100% of the light signal to digital C-mount cameras, letting pathology technicians record high-resolution live video and still images for patient records.
- Solid-state LED excitation eliminates expensive replacement bulbs and mercury arc disposal
- Belt-driven stage mechanism prevents hand injuries and gear wear over long shifts
- Selectable beam splitter allows 100% light transfer to digital cameras for low-noise capture
Optika Ten-Year Manufacturer Limited Warranty
Optika backs the B-293LD1 with an industry-leading 10-year warranty on mechanical and optical components, covering internal LED circuitry and dimmer controls for two years. The complete factory package contains the trinocular frame tower, Blue excitation filter set, four IOS N-PLAN lenses, immersion oil bottle, universal power adapter, dust wrap, and user manual.
Optika B-293LD1 Fluorescence Trinocular Microscope LED-FLUO N-PLAN IOS 1000x Blue Filterset Specs
| Brand | Optika |
| Full Model Code / SKU | B-293LD1 |
| Country of Origin | Italy |
| Microscope Type | Advanced Biological Epi-Fluorescence Trinocular Compound Microscope with Integrated Blue LED Excitation |
| Optical System Type | IOS Infinity Corrected Optical System designed to maintain complete optical purity along the internal light path |
| Observation Tube Type | Trinocular observation assembly combining dual visual ocular ports with an overhead vertical photo-imaging line |
| Tube Inclination & Rotation | Visual viewing tubes inclined at a fixed 30-degree position with a full 360-degree rotational mounting assembly |
| Eyepiece / Field of Number | Pair of widefield high-eyepoint WF10x eyepieces displaying an expansive field index of exactly 20 mm |
| Nosepiece Design | Quadruple revolving nosepiece operating on internal precision ball-bearings with structural click-stop positioning |
| Objectives Classification | IOS N-PLAN Infinitely Corrected Flat-Field Plan-Achromatic Lenses optimized for high-transmission fluorescence fields |
| Objectives Magnification Breakdown | Includes four primary objectives: IOS N-PLAN 4x, 10x, 40x (Spring), and an oil-immersion 100x (Spring) cartridge |
| Total Magnification Range | Provides discrete compound surface magnification tracking checkpoints at 40x, 100x, 400x, and up to 1000x points |
| Focusing Type & Graduation | Coaxial coarse and fine focus tracking control wheels; fine focus wheel increments at exactly 0.002 mm graduations |
| Stage Dimensions & Surface Coating | Double-layer belt-driven mechanical sliding specimen stage measuring 150 x 139 mm with a wear-resistant black finish coat |
| Stage Travel Range & Controls | Coaxial drop-down X-Y directional adjustment tracks delivering an active cross-travel span of exactly 75 x 33 mm |
| Condenser Type & Numerical Aperture | Abbe condenser tracking a 1.25 N.A. value, equipped with an integrated adjustable iris diaphragm assembly |
| Illumination Transmitted Type | Patented X-LED3 illumination module featuring an ultra-bright 3.6W white LED system with specialized collectors |
| Illumination Epi-EPI/Reflected | High-output 3.5W Blue LED Epi-fluorescence attachment (Excitation wavelength exactly 450-490 nm) |
| Light Intensity Management | Independent electronic dimming controls for separate transmitted white LED and reflected fluorescence light lines |
| Camera Port / C-Mount Adapter Split | Dedicated vertical photo port channel featuring a variable light path beam split mechanism (100% visual / 100% photo) |
| Contrast Methods Capability | LED Epi-fluorescence via Blue filter attachment, high-resolution transmitted brightfield analysis loops |
| Power Supply & Voltage Input | External switching multi-voltage international power pack module operating on 100-240V AC lines |
| Physical Dimensions & Net Weight | Total structural frame height 420 mm, base width 235 mm, depth 290 mm, assembly structural mass exactly 7.5 kg |
| Standard Package Accessories Included | Trinocular frame tower, Blue excitation filter set, four infinity plan lenses, power cable, system dust wrap |
| Primary Application & Environment | Tuberculosis auramine screening workstations, university cellular biology facilities, clinical biology instruction desks |




















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